Understanding spatial organization of membraneless organelles using proteomics approaches

As a postdoctoral fellow in Dr. Anne-Claude Gingras’ lab, I utilized in vivo proximity-dependent biotinylation assay (BioID) in human cells to systematically survey the proteome architecture of two cytosolic membraneless organelles, Stress Granules and P-bodies, and define their proteome (Mol. Cell; PMID: 29395067). Building upon this work, I propose to further investigate the molecular interactions governing the organization and the dynamic properties of Stress Granules, which are implicated in neurological disorder such as Amyotrophic Lateral Sclerosis (ALS).