Dr. Mu He
Postdoctoral Fellow, Lily Jan's Lab, UCSF
Monday, January 20, 2020 - 3:00pm
PGCRL Rm 1, 2nd Fl, 686 Bay St., SickKids
Faculty Recruitment Seminar
The conducting airway for air passage forms a protective mucosal barrier and presents the primary target of various airway disorders. Previous studies utilizing single cell RNA sequencing (scRNAseq) have systematically characterized airway cell types in adults and lineage networks within the developed airway in normal and chronic inflammatory conditions. Whether the same programs for airway regeneration in adults are also operating during embryogenesis, however, remains an open question. To better understand how the airway developmental programs are established to support air breathing and the barrier functions in newborns, we constructed a single-cell atlas of the human and mouse developing airway. Our data reveal hitherto unrecognized heterogeneity of cell states with distinct differentiation programs and immune features associated with the airway epithelium. Ciliated cells, one of the major cell types found in the airway epithelium, form motile cilia and generate efficient ciliary flow essential for mucus clearance. From our transcriptomic analysis, we identified gene modules associated with embryonic ciliated cells and postnatal ciliated cells, as well as a ciliated precursor state conserved in mice and humans. Furthermore, we identified a cell cluster exhibiting two sets of gene modules, the Foxj1 associated cilia module and the Gp2 associated secretory module. This cell state has not been observed in adult airway during homeostasis and regeneration. In parallel, we characterized a mouse mutant that shows altered epithelial cell landscape and multiple airway defects, including mucous cell hyperplasia and abnormal mucociliary clearance. Our data suggest that efficient mucociliary clearance depends on both ciliated cells and secretory cells. Together, our study illuminates unique programs for mammalian airway development and present a tractable mouse model for understanding the cellular processes required for airway differentiation and mucosal barrier function.
Drs. Brian Ciruna and John Brumell
Developmental and Stem Cell Biology