Mapping Genetic Interaction Networks in Yeast and Human Cells

Our group generated a comprehensive genetic network in yeast cells, testing all possible 18 million gene pairs for genetic interactions. Negative interactions connected functionally related genes and coherent sets of genetic interactions connect protein complex and pathway modules to map a functional wiring diagram of the cell. We’ve examined trigenic interaction networks and how different environments modulate the global yeast genetic interaction network. We are now exploring how different genetic backgrounds influence the global yeast genetic network. To test for conservation of the general principles of genetic networks, we are utilizing CRISPR-Cas9 technology to conduct genome-wide screens and map genetic interactions in haploid human cells.