Prof. Bengang Xing PhD.
Division of Chemistry and Biological Chemistry; School of Physical and Mathematical Sciences; Nanyang Technological University, Singapore
Wednesday, September 13, 2017 - 10:00am
PMH, 610 University Avenue, 7th Floor, Rm 7-605
Cutting Edge Fluorescence Imaging Strategies for Precise Regulation of Cellular Functions and Localized Theranostics Bengang XINGa,b* Division of Chemistry and Biological Chemistry; School of Physical and Mathematical Sciences; Nanyang Technological University, Singapore Email: Bengang@ntu.edu.sg http://www.ntu.edu.sg/home/bengang/index.htm In terms of the native 3D cell structures in all living species, the precise tracking of complexity and dynamics of cellular functions with precise resolution in intact cells or organisms will be important to understand the biological basis and to conduct related biomedical applications. Usually, within complex environment, biological specificity can be mediated by the precise regulation of biomolecule functions in response to intrinsic development programs and extrinsic signals. In order to fully decipher the biomolecule functions and further elucidate their mechanisms, a simple and reliable assay with high sensitivity and fidelity will be essential to efficiently report the biological process of interest at the single cell or molecular level. Generally, fluorescent imaging enables such rapid, direct and sensitive visualization, mainly due to their high sensitivity, relative safety, and easily handling, and therefore have become robust and reliable tools in monitoring subcellular protein dynamics and analysis of tumors or pathogen–host interactions. The systematic imaging investigation of enzymes or proteins activities in a complicated environment will offer great possibility for the in-depth understanding of the biological basis conferring diseases status, and importantly, for the facilitating of new discovery of effective theranostic approaches in vitro and in vivo. In our group, a series of simple and specific fluorescent/bioluminescent small molecules or nano-probes have been extensively established to real-time visualize cellular functions, importantly, the intrinsic mechanisms to involve in potent drug activities and relevant pathways to initiate drug resistance have been well investigated. References:  Ai, X.; Ho, C.-J.; Aw, J.; Attia, A.-B.; Mu, J.; Wang, Y.; Wang, X.; Wang, Y.; Liu, X.; Chen, H.; Gao, M.; Chen, X.; Yeow, K; Liu, G.*; Olivo, M.*; Xing, B.* Nat. Commun. 2016, 7: 10432. Featured in “Biodiscover”, “ScienceNet”, and “X-MoL”.  Min, Y.; Li, J.; Liu, F.; Yeow, K.*; Xing, B.* Angew. Chem. Int. Ed. 2014, 53: 1012.  Ai, X.; Lyu, L.; Zhang, Y.; Tang, Y.; Mu, J.; Liu, F.; Zhou, Y.; Zuo, Z.; Liu, G.*; Xing, B.* Angew. Chem. Int. Ed. 2017, 56: 3031. Featured as “Backcover” and Highlighted in “Advanced Science News, Wiley” and “X-MoL”.  Yang, Y.; Shao, Q.; Deng, R.; Wang, C.; Teng, X.; Cheng, K.; Cheng, Z.; Huang, L.; Liu, Z.; Liu, X.*; Xing, B.* Angew. Chem. Int. Ed. 2012, 51: 3125. Featured as “Hot Paper” and “Inside Cover”.  Mu, J.; Liu, F.*; Rajab, M.-S.; Shi, M.; Li, S.; Goh, C.; Lu, L.; Xu, Q.-H.; Liu, B.; Ng, L.-G.; Xing, B.* Angew. Chem. Int. Ed. 2014, 53: 14357.
Dr. Gang Zheng
Princess Margaret Special Seminar