Dr. Alexander Dunn
Department of Chemical Engineering, Stanford University
Wednesday, September 9, 2015 - 3:00pm
PG CRL Rm 1, 2nd Fl, 686 Bay St., SickKids
Invited Speaker Seminar
Abstract:
Understanding how hundreds to millions of cells work together to form or repair tissues and
organs represents a central challenge in cell and developmental biology. Recent studies
demonstrate that tissue growth and patterning are inherently physical processes, and that
individual cells are exquisitely sensitive to stretch, fluid flow, and extracellular matrix (ECM)
stiffness. However, the molecular mechanisms by which living cells sense mechanical stimuli
remain poorly understood. In this talk I describe our work to understand the protein-based
molecular machines that cells use to sense and transduce mechanical force at cell-cell and cellmatrix
adhesions. We used a single-molecule optical trap assay to determine a probable
mechanism by which cells sense mechanical stretch at cell-cell contacts, a physical cue that is
thought to be central in controlling growth and patterning in living tissues (Buckley et al.,
Science, 2014). In related work, we developed fluorescent molecular tension sensors to
visualize the nanometer-scale structures that link cells to the extracellular matrix (Morimatsu et
al., Nano Lett. 2015). We find that individual integrins exert relatively modest forces, consistent
with a collective model for cellular force generation and force sensing. These observations,
together with those from projects investigating the biophysical basis for the sense of touch
(Krieg et al., Nat. Cell Biol., 2014) and cell motility in three-dimensional matrices, suggest deep
underlying commonalities in how cells may detect and respond to mechanical force in a wide
variety of physiological circumstances.
Host:
Dr. Sevan Hopyan
Developmental and Stem Cell Biology