In situ imaging of germline stem cell division and architecture during C. elegans development

Stem cells offer great therapeutic promises for a number of degenerative diseases, yet their use requires that we obtain a comprehensive knowledge of their biology. While ground-breaking work has led to understanding genetic networks that control a variety of stem cell types, few studies have elucidated the cell biological properties controlling stem cells in vivo. This is due to the fact that, in general, stem cell niche environments are inaccessible for direct, live observation. We have begun a characterization of stem cell properties by live-imaging of dividing C. elegans germline stem cells in situ. I will present our findings on the regulation of their mitotic properties and on their tissue organization during gonad development.